R4RIN.com – MCQs, Mock Tests | Biochemistry Biochemistry -Immunological Techniques

1. Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
2000 kb and 20kb DNA
1000 kb and l0kb DNA
20 kb and 2000 kb DNA
20 kb and 2000 kb DNA

2. The IPTG is a gratuitous inducers of (β-galactosidase and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?
Blue
White
Brown
No colour is produced

3. The competitive immunoassay can be used
to detect very small amounts of antigen
to detect antibody associated with allergies (IgE)
both (a) and (b)

4. Which of the following is an immunodiffusion test?
Double-diffusion
Gel diffusion
Ouchterloney technique
All of these

5. In Counter immunoelectrophoresis
electrophoresis will drive the antibody and antigen parallel to each other
electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards anode
the antibody will migrate towards cathode

6. What is the ELISA test intended to measure?
Antibody to HIV only
Antigen to HIV only
Presence of free circulating virus in the patient
Antibodies directed against HLA molecules

7. Immunoaffinity chromatography can be used in biochemical applications to
break down antibody structure
purify protein antigen
break down antigen and analyze quantitatively
none of the above

8. Different form of a plasmid on an agarose gel after electrophoresis may be in which of the following sequence in decreasing molecular weight?
Open circular super coiled linear plasmid DNA
Linear plasmid open circular super coiled plasmid
Open circular super coiled linear plasmid DNA
commonly to detect trace amounts of drugs.

9. Radial Immunodiffusion is similar to
double-diffusion
gel diffusion
Ouchterloney technique
all of these

10. In Rocket Immunodiffusion the length of the rocket is
proportional to the amount of antibody placed in each well
inversely proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well
proportional to the amount of antigen placed in each well