A branch of botany is
1. Genetic engineering
2. Plant Tissue culture
3. Plant Physiology
4. Plant ecology
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An example of a fusogenic agent is
1.Methanol
2. Ethyl alcohol
3. Polyethelene alcohol
4. Polyethelene glycol (PEG)
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Bacillus subtilis and streptomyces spp. are Prokaryotes which are used as hosts for introducing
1.Plasmid
2.Replicon
3.Auxin
4.rDNA
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Cells of higher plants are grown artificially in laboratory conditions using the technique of
1. Molecular cloning
2. Gene cloning
3. Tissue culture
4. Hybridzation
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Cellulase and Cellulysin are the cell wall degrading enzyme mixtures which can digest
1. Cell wall
2. Epidermal cells
3. Mesophyll
4. Xylem vessels
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DNA ligase was discovered in
1.1906
2.1910
3.1966
4.1970
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DNA of donor is cut into pieces using
1. Vector
2. Plasmid
3. Endonuclease
4. Inoculum
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DNA recombinant technology is also known as
1.Gene manipulation
2.Totipotency
3.Splicing
4.Gene cloning
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Each colony is a collection of bacteria having many copies of rDNA fragments called
1. Molecular cloning
2.Splicing
3.Cloning vehicles
4.Vectors
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Flamed and cooled platinum wire is used to transfer
1.Bacteria
2.Fungi
3.Callus
4.Inoculum
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Foot and mouth disease of cattle is cured by
1. Insulin
2.Cytokinin
3. Vaccine
4.Interferon
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Genes are directly introduced into the eukaryotie host for cloning through a
1.Plasmid
2. Vector
3. Endonuclease
4. Hormone
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Genetic engineering started with flying colours in the early
1.1900
2.1950
3.1960
4.1970
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If the DNA fragments of donor do not replicate in the host they are attached to a suitable
1. DNA ligase
2.Plasmid
3.Splicing
4.Replicon
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Industrial production of enzymes vitamins vaccines and bio-fertilizers are the products of
1.Ploidy
2.Genetics
3.Physiology
4.Biotechnology
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Inoculated culture vessels are protected from contamination of other organism by
1.Cleaning
2. Cotton plug
3. Oil cloth
4. Cork plug
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Inter specific crosses in industrial micro-organisms are made easier by
1. Protoplast fusion
2. Tissue culture
3. Genetic engineering
4.Totipotency
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Introducing the nitrogen fixing genes from bacteria to major food crops is possible through
1. Tissue culture
2.Hybridization
3. Protoplast
4. Genetic engineering
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Joining of donor DNA fragments and vector DNA fragments with the help of DNA ligase enzyme is known as
1. Gene cloning
2.Splicing
3.Gene manipulation
4.Molecular cloning
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Molecular cloning is also known as
1. Splicing
2.Gene cloning
3.Gene manipulation
4.Hardening
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Plants obtained through tissue culture method constitute genetically uniform population called
1. Clone
2. Callus
3.Inoculum
4.Hardening
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Protoplasmic fusion is of great importance in plants which are incompatible to
1. Molecular cloning
2. Gene cloning
3.Hybridization
4. Tissue culture
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Replicons are also known as
1.Ligase
2.Plasmids
3.Auxin
4.Vectors
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Restriction endonucleases are discovered in
1.1910
2.1906
3.1966
4.1970
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Several plantlets are developed from the
1. Clone
2.Inoculum
3.Callus
4.Plasmid
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Somatic hybridization is brought about by employing the technique of
1.Tissue culture
2.Splicing
3.Totipotency
4.Hardening
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Suitable host in genetic engineering to introduce DNA fragments of donor is
1.Yeast
2. Bacillus subtilis
3. Escherichia coli
4. Bacteriophages
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The ability of every living plant cell to produce the entire plant is called
1. Plasmid
2. Protoplast
3. Gene cloning
4. Totipotency
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The agent which promotes the fusion of protoplasts is called
1. Mutagenic agent
2. Fusogenic agent
3. Chemical agent
4. Cellulase
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The antiviral protein produced by virus infected cells is called
1.Insulin
2. Auxin
3. Vaccine
4. Interferon
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The cells in tissue culture divide and form a mass of undifferentiated tissue called
1.Inoculum
2. Plasmid
3. Replicon
4. Callus
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The contaminated microorganism in culture medium is
1.Fungi
2.Algae
3.Callus
4.Cytokinin
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The culture medium is prepared from
1. Proteins
2.Vitamins
3. Agar
4.Bacteria
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The DNA of vector is cut into fragments by
1.plasmid
2. Auxin
3. Restriction endonucleases
4. Cytokinin
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The donor DNA fragments and vector DNA fragments are joined together by
1.DNA ligase
2.Plasmid
3.Auxin
4.Cytokinin
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The enzyme which acts like a paste molecule to join DNA fragments
1.Plasmid
2.Clone
3. DNA ligase
4.Protease
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The extra-chromosomal circular DNA found in the E.coli is
1.Plasmid
2. DNA ligase
3.Vector
4. Plasmid
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The first step in protoplasmic fusion is the
1. Isolation of protoplasts
2. Fusion of protoplasts
3. Growth protoplasts
4. Regeneration of cell wall
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The following is not a branch of biotechnology
1.Genetic engineering
2.Tissue culture
3.Physiology
4.Protoplast fusion
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The following is used in industrial fermentation to produce food and drinks
1.Yeast
2.Algae
3.Vitamins
4.Vaccines
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The following ratio of hormonal concentration is required to induce callus to form shoot and root
1.Gibberellins to auxin
2. Auxin to cytokinin
3. Gibberellins to cytokinin
4. Ethylene to auxins
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The host bacterium follows the instruction of the 2
1.DNA ligase
2.Foreign rDNA
3.Plasmid
4.Auxin
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The inherent capacity of an isolated plant cell to give rise to a whole plant is called
1. Totipotency
2. Protoplast
3. Hybridization
4. Gene manipulation
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The instrument used for keeping inoculated vessels at a desired constant temperature is
1. Platinum wire
2.Micropipette
3. Petriplate
4. Incubator
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The instrument used to transfer inoculums under aseptic condition is
1.Incubator
2. Culture tube
3. Micropipette
4. Petriplate
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The interspecfic somatic hybrids of tobacco and petunia are produced by the technique of
1. Tissue culture
2. Protoplasmic fusion
3. Genetic engineering
4. Gene cloning
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The isolated protoplasts are grown in
1.Hypotonic culture medium
2. Hypertonic culture medium
3. Isotonic culture medium
4. Agar culture medium
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The isolated protoplasts are made to fuse to produce a hybrid cell in
1. Tissue culture
2. Genetic engineering
3.Protoplast fusion
4. Gene cloning
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The mechanism which has the ability to engineer new organisms is known as
1.Totipotency
2.Molecular cloning
3.Genetic engineering
4.Splicing
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The molecular scissors which cut DNA at specific sites are
1. Plasmids
2. Fusogenic agents
3.Inoculum
4. Restriction enzymes
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The most suitable vectors are
1.Yeast
2.Bacteriophages
3.Moulds
4.Algae
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The other name for hybrid DNA is
1.Recombinant DNA (rDNA)
2. DNA ligase
3.Plasmid
4.Replicon
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The plant cell walls are digested by specific cell wall degrading
1. Protein mixtures
2. Vitamin mixtures
3.Enzyme mixtures
4. Hormonal mixtures
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The process of transferring plantlets to pots and subjected gradually to natural environmental conditions is called
1. Hardening
2.Totipotency
3.Cloning
4.Splicing
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The production of insulin interferon human growth hormones proteins and vaccines are based on
1. Tissue culture
2. Protoplast fusion
3. Genetic engineering
4. Hybridization
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The production of new characteristics by introducing new genes and altering the genome is known as
1.DNA recombinant technology
2. Protoplast fusion
3.Totipotency
4.Splicing
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The rapid and cheaper method of propagating plants with desire qualities is
1. Molecular cloning
2. Gene cloning
3. Tissue cloning
4. Genetic cloning
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The rDNA is introduced into this host
1. E.coli
2.Yeast
3.Mould
4.Algae
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The recent and fascinating branch of biology is
1. Physiology
2.Genetics
3. Genetic engineering
4.Ecology
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The rigid plant cell wall is digested by
1.Pectinase
2. Protease
3. Amylase
4.Sucrase
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The technique used industrially to multiply plants which are difficult to propagate by conventional means is
1. Protoplast fusion
2. Tissue culture
3.Hybridization
4. Genetic engineering
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The technique which is useful in genetic engineering to introduce rDNA and other DNA fragments into higher plant cells in culture is
1. Protoplasmic fusion
2. Tissue culture
3. Hardening
4.Splicing
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The transfer of inoculums is done under
1. Aseptic condition
2. Septic condition
3. Hygenic condition
4. Environmental condition
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The two different genetic sources protoplasts usually used are
1.Mesophyll and parenchyma cells
2.Mesophyll and epidermal cells
3. Epidermal cells and parenchyma cells
4.Hypodermal and mesophyll cells
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Tissue culture technique is successfully employed to bring about
1. Protoplasmic fusion
2. Gene manipulation
3. Splicing
4. Hardening
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Vectors are also known as
1.DNA ligase
2.Plasmid
3. Cloning vehicles
4. Inoculum
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Very small number of isolated living cells is called
1.Plasmid
2. Vector
3. Auxin
4. Inoculum
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Virus free potatoes tulips sugarcanes and orchids are obtained by the technique of
1.Hardening
2. Totipotency
3. Tissue culture
4. Splicing
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Yoghurt is a food product produced by employing
1.Hybridization
2.Tissue culture
3.Protoplast fusion
4.Biotechnology
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